3M Attest Rapid Readout Sterilization Biological Indicator Vial Steam

Brand/Manufacturer: 3M
Item # Desc Pkg Price
1291 Time To Results: 1 Hour Read Time $6.93
1291 Time To Results: 1 Hour Read Time $26.77
1292 Time To Results: 3 Hour Read Time $6.93
1292 Time To Results: 3 Hour Read Time $26.77

3M Attest Rapid Readout Biological Indicator For Steam is a dual readout biological indicator system specifically designed for rapid and reliable monitoring for 121-123°C Gravity and 132-135°C Vacuum-Assisted steam sterilization processes when used in conjunction with the 3M Attest Auto-reader 290 or the 3M Attest Auto-reader 390.


  • 3M Attest Rapid Readout Biological Indicator For Steam detects the presence of Geobacillus stearothermophilus by detecting the activity of alpha-glucosidase, an enzyme present within the organism
  • Presence of the enzyme is detected by reading fluorescence produced by the enzymatic breakdown of a non-fluorescent substrate, this creates a fluorescence change, which is detected by the auto-reader
  • Fluorescence change indicates a steam sterilization process failure 
  • Presence of G. stearothermophilus organisms by a visual color change reaction
  • Biochemical activity of the G. stearothermophilus organism produces acid by-products that cause the media to change color from purple to yellow
  • Visual pH color change also indicates a steam sterilization process failure
  • Due to the high sensitivity of the 3-hour fluorescent results, however, there is no advantage to incubating the Attest 1292 Rapid Readout Biological Indicator beyond 3 hours
  • Provides results in three hours
  • Compliant with ANSI/AAMI/ISO 11138:2006 Parts 1 and 3
  • Self-contained, reducing the risk of false positives due to contamination
  • Automatic readout means unambiguous YES/NO, PASS/FAIL result
  • Indications: Use the Attest™ 1292 Rapid Readout Biological Indicator to monitor:
    - 250°F (121°C) gravity steam sterilization cycles
    - 270°F (132°C) vacuum assisted steam sterilization cycles
  • Click Here for User Manual

More Information

  • Storage: 
    - Best stored in the original box under normal room conditions: 15-30ºC (59-86ºF) and 35-60% relative humidity.
    - Do not store these biological indicators near sterilants or other chemicals. 
  • Precautions:
    Do not use the Attest™ 1292 Rapid Readout Biological Indicator to monitor:
    - 270°F (132°C) gravity steam sterilization cycles.
    - Dry heat, chemical vapor, ethylene oxide or other low temperature sterilization processes. 
  • Warning: 
    - There is a glass ampoule inside the plastic vial of the biological indicator. To avoid the risk of serious injury from flying debris due to a ruptured biological indicator:
    - Allow the biological indicator to cool for the recommended time period before crushing. Crushing or excessive handling of the biological indicator before cooling may cause the glass ampoule to burst.
    - Wear safety glasses and gloves when removing the biological indicator from the sterilizer.
    - Wear safety glasses when crushing the biological indicator.
    - Handle the biological indicator by the cap when crushing and tapping.
    - Do not use your fingers to crush the glass ampoule.
    - Do not roll the biological indicator between fingers to wet the spore strip

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What is HPFY’s return policy?

Customer satisfaction is our top priority. We stand behind our products. If the merchandise you received is defective or not what you expected it to be, and you wish to return the item, please submit a return request in the My Account section of our website, contact our Customer Service department at (866) 316-0162 / (203) 616-2850 or email us.

  1. Merchandise must be returned within 30 days of the receipt of your order. Returns are not accepted after 30 days.
  2. Items (including parts and accessories) must be returned in new, unused, and resalable condition in its original packaging.
  3. Before returning any product, the buyer must obtain a Return Authorization Number (RA#) from a customer service representative. With your RA# you will receive a prepaid return shipping label.
  4. Orders returned without obtaining an RA# will be entitled to receive only "In-Store Credit" that can be used towards future purchases.
  5. Return orders are subject to a 20% restocking fees.
  6. All custom orders and hygienic items are non-returnable. Refer to the “Non-Returnable Items” section for further details.
  7. Returns will be reviewed and inspected before issuing credit. Please allow 3 to 4 weeks for processing. The credit will be applied to your original method of payment.
  8. If the return is the result of a defective product or shipping error, we will refund the entire purchase amount and return shipping costs.

Which products are Non-Returnable?

Due to the hygienic nature of some items, they are non-returnable. The items listed below are non-returnable:

  1. All hygienic items.
  2. Opened personal care items.
  3. Anything opened, used, or tried on.
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  5. Besides these, items that have the “non-returnable” phrase on the website.

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How do I receive a return authorization number?

You can submit a return request by logging into your account. You will receive the Return Authorization Number (RA#) details in the email within 2-3 business days.

  • Login to your account
  • Go to “order history”
  • Click on “return order”
  • Fill out the return form and Submit it

If you do not have an email address, we will make other accommodations for RA# Details. Write the RA# on your label affixed to your package prior to shipment.

How long does it take to receive a refund?

Please allow 3 to 4 weeks to process the returns. Once the item is received, and inspected, your refund will be processed and automatically applied to your credit card or original method of payment.

Is there any Restocking Fees?

  1. 20% restocking fees will be applied.
  2. Outbound shipping cost is non-refundable.
  3. For orders that qualified for free shipping, $8.99 will be deducted from the refund if the product is returned.

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